编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

SME Medium (DSMZ Medium 1155)

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

SME Medium

(DSMZ Medium 1155)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ........................................................................................... 27.7g

Sulfur, elemental ......................................................................... 10.0g

MgSO4·7H2O ................................................................................ 7.0g

MgCl2·6H2O.................................................................................. 5.5g

KH2PO4......................................................................................... 1.0g

CaCl2·2H2O................................................................................. 0.75g

KCl ............................................................................................. 0.66g

(NH4)2SO4..................................................................................... 0.5g

NaBr ........................................................................................... 0.10g

H3BO3 ......................................................................................30.0mg

SrCl2·6H2O...............................................................................15.0mg

Sulfide solution ........................................................................20.0mL

Yeast extract solution...............................................................10.0mL

KJ solution ...............................................................................0.05mL

pH 5.5 ± 0.2 at 25°C

KJ Solution:

Composition per 10.0mL:

KJ............................................................................................. 10.0mg

Preparation of KJ Solution: Add KJ to distilled/deionized water

and bring volume to 10.0mL. Mix thoroughly.

Sulfide Solution:

Composition per 10.0mL:

Na2S·9H2O.................................................................................. 0.25g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave

under 100% N2 for 15 min at 15 psi pressure–121°C. Cool to room

temperature.

Yeast Extract Solution:

Composition per 10.0mL:

Yeast extract.................................................................................. 0.4g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except sulfide and

yeast extract solutions, to distilled/deionized water and bring volume

to 970.0mL. Mix thoroughly. Gently heat and bring to boiling. Sparge

with a gas mixture of 80% H2 + 20% CO2. Autoclave for 15 min at 15

psi pressure–121°C. Cool to room temperature. Aseptically add sulfide

solution. Mix thoroughly. Adjust pH to 5.5 with sulfuric acid. Asepti

cally add yeast extract solution. Mix thoroughly. Sparge with a gas

mixture of 80% H2 + 20% CO2. Aseptically distribute into culture ves

sels.

Use: For the cultivation of Thermodiscus maritimus.

在线客服

SME Medium (DSMZ Medium 1155)