编号：
批号：

培养基配方

您现在的位置：网站首页 >> 培养基配方

SME Medium, Modified

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

NaCl............................................................................................ 30.0g

MgSO4·7H2O................................................................................ 7.0g

NaHCO3........................................................................................ 2.0g

KCl.............................................................................................. 0.65g

CaCl2·2H2O .................................................................................. 0.5g

K2HPO4....................................................................................... 0.15g

NH4Cl ......................................................................................... 0.15g

NaBr.............................................................................................. 0.1g

MgCl2·6H2O .............................................................................. 5.5mg

Trace elements solution ...........................................................10.0mL

pH 6.5 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O............................................................................... 3.0g

(NH4)2Ni(SO4)2 ............................................................................ 2.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl.............................................................................................. 1.0g

MnSO4·2H2O............................................................................... 0.5g

CaCl2·2H2O .................................................................................. 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O............................................................................... 0.025g

KAl(SO4)2·12H2O....................................................................... 0.02g

CoSO4·7H2O............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CuSO4·5H2O............................................................................... 0.01g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·2H2O ......................................................................... 0.01g

Na2WO4 ...................................................................................10.0mg

Na2SeO4 ...................................................................................10.0mg

Na2SeO3·5H2O...........................................................................0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to approximately 500.0mL of distilled/deionized water. Dissolve

by adding KOH and adjust pH to 6.5. Add remaining components.

Bring volume to 1.0L with additional distilled/deionized water. Adjust

pH to 7.0 with KOH.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L.Mix thoroughly. Sparge with 100% N2

for 20 min. Adjust pH to 6.5–6.8 with H2SO4. Distribute 10.0mL vol

umes into 120.0mL serum bottles while gassing under 100% CO2.

Stopper each serum bottle tightly. Exchange the gas phase in each se

rum bottle with 79.75% H2 + 19.75% CO2 + 0.5% O2 and bring pres

sure to 300KPa. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Aquifex pyrophilus.

在线客服

SME Medium, Modified