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SME Medium Modified for IS7 (DSMZ Medium 891)

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

SME Medium Modified for IS7

(DSMZ Medium 891)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 30.0g

NaHCO3 ........................................................................................ 2.0g

MgSO4·7H2O ................................................................................ 0.7g

KCl.............................................................................................. 0.65g

MgCl2·6H2O................................................................................ 0.55g

CaCl2·2H2O................................................................................... 0.5g

Sulfur, powdered........................................................................... 0.5g

NH4Cl ......................................................................................... 0.15g

K2HPO4....................................................................................... 0.15g

NaBr.............................................................................................. 0.1g

Trace elements solution ...........................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O ................................................................................ 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl .............................................................................................. 1.0g

MnSO4·2H2O ................................................................................ 0.5g

CoSO4·7H2O ............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O................................................................................... 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O ............................................................................... 0.025g

KAl(SO4)2·12H2O....................................................................... 0.02g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·4H2O .......................................................................... 0.01g

CuSO4·5H2O ............................................................................... 0.01g

Na2SeO3·5H2O...........................................................................0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH

to 6.5 with KOH. Add remaining components. Add distilled/deionized

water to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 us

ing H2SO4. Distribute into anaerobe tubes or bottles. Autoclave for 15

min at 15 psi pressure–121°C. Provide an atmosphere of 78% H2 +

20% CO2 + 2% O2.

Use: For the cultivation of unclassified bacterium DSM 12047.