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Sodium Chloride Sucrose Medium 900 with Penicillin G

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Sodium Chloride Sucrose Medium 900

with Penicillin G

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................ 97.3g

Pancreatic digest of gelatin ......................................................... 14.5g

NaCl ............................................................................................ 14.3g

Agar ............................................................................................ 13.3g

Brain heart, solids from infusion .................................................. 6.0g

Peptic digest of animal tissue........................................................ 6.0g

Yeast extract.................................................................................. 5.0g

Glucose ......................................................................................... 3.0g

Na2HPO4 ....................................................................................... 2.5g

MgSO4 ........................................................................................ 0.25g

Horse serum (γ-globulin free,

inactivated 30 min at 56°C).........................................100.0mL

Penicillin solution ....................................................................10.0mL

pH 7.4 ± 0.2 at 25°C

Penicillin Solution:

Composition per 10.0mL:

Penicillin G .......................................................................... 500,000U

Preparation of Penicillin Solution: Add penicillin G to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Preparation of Medium: Add components, except penicillin solu

tion and horse serum, to distilled/deionized water and bring volume to

900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

add penicillin solution and horse serum. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Pseudomonas aeruginosa.