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Sodium Dodecyl Sulfate Polymyxin Sucrose Agar

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Sodium Dodecyl Sulfate

Polymyxin Sucrose Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 20.0g

Agar ............................................................................................ 15.0g

Sucrose........................................................................................ 15.0g

Proteose peptone ......................................................................... 10.0g

Beef extract ................................................................................... 5.0g

Sodium lauryl sulfate .................................................................... 1.0g

Bromthymol Blue ....................................................................... 0.04g

Cresol Red................................................................................... 0.04g

Polymyxin B solution ..............................................................10.0mL

pH 7.6 ± 0.2 at 25°C

Polymyxin B Solution:

Composition per 10.0mL:

Polymyxin B sulfate............................................................. 100,000U

Preparation of Polymyxin B Solution: Add polymyxin B sulfate

to distilled/deionized water and bring volume to 10.0mL. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components, except polymyxin B

solution, to distilled/deionized water and bring volume to 990.0mL.

Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile

polymyxin B solution. Mix thoroughly. Pour immediately into sterile

Petri dishes or distribute into sterile tubes.

Use: For the isolation and cultivation of Vibrio species from foods.