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Sodium Hippurate Broth (Hippurate Broth)

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Sodium Hippurate Broth

(Hippurate Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Beef heart, solids from infusion................................................ 500.0g

Tryptose ...................................................................................... 10.0g

Sodium hippurate........................................................................ 10.0g

NaCl.............................................................................................. 5.0g

pH 7.4 ± 0.2 at 25°C

Source: Heart infusion broth is available as a premixed powder from

BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into screw-capped tubes or flasks. Autoclave for

15 min at 15 psi pressure–121°C. Tighten caps to prevent drying.

Use: For the identification and differentiation of β-hemolytic strepto

cocci based on hippurate hydrolysis. After inoculation and incubation,

tubes are treated with FeCl3 reagent. A heavy precipitate remaining

after 10–15 min indicates that hippurate has been hydrolyzed.