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Soil Extract Agar

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Soil Extract Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Soil............................................................................................ 500.0g

Agar ............................................................................................ 15.0g

Glucose ......................................................................................... 2.0g

Yeast extract.................................................................................. 1.0g

KH2PO4......................................................................................... 0.5g

Preparation of Medium: Add 500.0g of garden soil to 1.0L of tap

water. Autoclave for 3 hr at 15 psi pressure–121°C. Filter through

Whatman #2 filter paper. Add remaining components to filtrate. Bring

volume to 1.0L with tap water. Gently heat and bring to boiling. Dis

tribute into tubes in 7.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C. Allow tubes to cool in a slanted position.

Use: For the cultivation and identification of Histoplasma capsulatum,

Blastomyces dermatitidis, and Bacillus species based on the formation of

typical conidia.