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Soil Extract Agar

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Soil Extract Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Soil extract....................................................................................1.0L

pH 6.8 ± 0.2 at 25°C

Soil Extract:

Composition per liter:

Soil............................................................................................ 400.0g

Preparation of Soil Extract: Air dry garden soil with a high con

tent of organic matter and pass through a sieve. Weigh out 400.0g and

add to 960.0mL of tap water. Autoclave for 60 min at 15 psi pressure–

121°C. Cool to room temperature and allow soil to settle out. Decant

supernatant solution. Filter through paper. Bring volume to 1.0L with

distilled/deionized water.

Preparation of Medium: Add agar to 1.0L soil extract. Gently heat

and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Aureobacterium flavescens and Bacillus

species.