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Soil Extract Medium

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

Soil Extract Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Soil............................................................................................ 400.0g

Agar ............................................................................................ 15.0g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Sieve air-dried garden soil with a high

content of organic matter. To 400.0g of soil add 1.0L of distilled/deion

ized water. Autoclave for 60 min at 15 psi pressure–121°C. Cool to

room temperature. Allow solids to sediment for a few hours. Carefully

decant the supernatant solution. Centrifuge the supernatant solution at

10,000 × g for 15 min. Decant supernatant solution. Add agar (15.0g

per liter of supernatant). Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Arthrobacter species, Aureobacterium fla

vescens, Aureobacterium terregens, and Bacillus thiaminolyticus.