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Soil Extract Peptone Beef Extract Medium

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Peptone ......................................................................................... 5.0g

Beef extract................................................................................... 3.0g

Soil extract....................................................................................1.0L

pH 7.0 ± 0.2 at 25°C

Soil Extract:

Composition per liter:

Garden soil................................................................................ 400.0g

Preparation of Soil Extract: Add garden soil to 1.0L of tap water.

Autoclave for 1 hr at 15 psi pressure–121°C. Filter through cheesecloth

and Whatman #2 filter paper. Autoclave filtrate again for 20 min at 15

psi pressure–121°C. Filter through Whatman #2 filter paper.

Preparation of Medium: Add agar, peptone, and beef extract to

1.0L of soil extract. Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Oerskovia turbata and

Oerskovia xanthineolytica.