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Soil Extract Potato Extract Medium

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 510.0mL:

Malt extract................................................................................. 10.0g

Yeast extract.................................................................................. 4.0g

Potato extract .........................................................................250.0mL

Soil extract.............................................................................250.0mL

pH 7.0 ± 0.2 at 25°C

Soil Extract:

Composition per liter:

Garden soil................................................................................ 400.0g

Preparation of Soil Extract: Add garden soil to 1.0L of tap water.

Autoclave for 45 min at 15 psi pressure–121°C. Filter through cheese

cloth.

Potato Extract:

Composition per liter:

Potatoes..................................................................................... 400.0g

Preparation of Potato Extract: Peel and dice potatoes. Add

500.0mL of distilled/deionized water. Gently heat and bring to boiling.

Continue boiling for 15 min. Filter through cheesecloth. Bring volume

to 1.0L with distilled/deionized water.

Preparation of Medium: Combine components. Mix thoroughly.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation and maintenance of Saccharopolyspora recti

virgula.