培养基配方

您现在的位置：网站首页 >> 培养基配方

Soil Extract Salts Medium

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Soil extract stock....................................................................625.0mL

Solution 1...............................................................................125.0mL

Solution 2...............................................................................125.0mL

Solution 3...............................................................................125.0mL

Soil Extract Stock:

Composition per liter:

Soil, air dried and sieved........................................................... 333.3g

Preparation of Soil Extract Stock: Add soil to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 8.0 with

1N NaOH or HCl. Autoclave for 30 min at 15 psi pressure–121°C. Al

low soil to settle out. Carefully pour off supernatant. Filter through two

layers of cheesecloth.

Solution 1:

Composition per liter:

K2HPO4......................................................................................... 1.0g

Preparation of Solution 1: Add K2HPO4 to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly.

Solution 2:

Composition per liter:

MgSO4·7H2O ................................................................................ 1.0g

Preparation of Solution 2: Add MgSO4·7H2O to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Solution 3:

Composition per liter:

KNO3 .......................................................................................... 10.0g

Preparation of Solution 3: Add KNO3 to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Combine components. Mix thoroughly.

Filter sterilize. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Chlamydomonas applanata, Polytoma uvella,

Polytoma mirum, Polytoma ellipticum, Polytoma difficile, and Polytoma

anomale.