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Soil Seawater Medium for Algae

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

HESNW medium solution .....................................................750.0mL

Soil extracts salts medium solution .......................................250.0mL

HESNW Medium Solution:

Composition per 1011.0mL:

Natural seawater ...........................................................................1.0L

Enrichment solution.................................................................10.0mL

Vitamin solution.........................................................................1.0mL

Preparation of HESNW Medium Solution: Allow natural seawa

ter to age for 2 months. Filter sterilize. Aseptically add 10.0mL of sterile

vitamin solution and 1.0mL enrichment solution. Mix thoroughly.

Enrichment Solution:

Composition per liter:

NaNO3 ...................................................................................... 4.667g

Na2SiO3·9H2O .......................................................................... 3.000g

Sodium glycerophosphate......................................................... 0.667g

EDTA·2H2O.............................................................................. 0.553g

H3BO3 ....................................................................................... 0.380g

Fe(NH4)2(SO4)2·6H2O .............................................................. 0.234g

MnSO4·4H2O............................................................................ 0.054g

FeCl3·6H2O............................................................................... 0.016g

ZnSO4·7H2O .............................................................................. 7.3mg

CoSO4·7H2O.............................................................................. 1.6mg

Preparation of Enrichment Solution: Add Na2SiO3·9H2O to dis

tilled/deionized water. Mix thoroughly. Neutralize Na2SiO3·9H2O with

1N HCl. Add 500.0mL of distilled/deionized water. Mix thoroughly.

Add remaining components and bring volume to 1.0L with distilled/

deionized water. Mix thoroughly. Filter sterilize.

Vitamin Solution:

Composition per liter:

Thiamine....................................................................................... 0.1g

Vitamin B12 ................................................................................ 2.0mg

Biotin ......................................................................................... 1.0mg

Preparation of Vitamin Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster

ilize.

Soil Extracts Salts Medium Solution:

Composition per liter:

Soil extract stock....................................................................625.0mL

Solution 1...............................................................................125.0mL

Solution 2...............................................................................125.0mL

Solution 3...............................................................................125.0mL

Soil Extract Stock:

Composition per liter:

Soil, air dried and sieved .......................................................... 333.3g

Preparation of Soil Extract Stock: Air dry soil. Sieve through

fine-mesh screen. Add soil to distilled/deionized water and bring vol

ume to 1.0L. Adjust pH to 8.0 with 1N NaOH or 1N HCl. Autoclave

for 30 min at 15 psi pressure–121°C. Allow soil to settle. Decant liquid.

Filter through cheesecloth.

Solution 1:

Composition per liter:

K2HPO4......................................................................................... 1.0g

Preparation of Solution 1: Add K2HPO4 to distilled/deionized wa

ter and bring volume to 1.0L. Mix thoroughly.

Solution 2:

Composition per liter:

MgSO4·7H2O................................................................................ 1.0g

Preparation of Solution 2: Add MgSO4·7H2O to distilled/deion

ized water and bring volume to 1.0L. Mix thoroughly.

Solution 3:

Composition per liter:

KNO3 .......................................................................................... 10.0g

Preparation of Solution 3: Add KNO3 to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly.

Preparation of Soil Extracts Salts Medium Solution: Com

bine 625.0mL of soil extract stock, 125.0mL of solution 1, 125.0mL of

solution 2, and 125.0mL of solution 3. Filter sterilize.

Preparation of Medium: Aseptically combine 750.0mL of sterile

HESNW medium solution and 250.0mL of sterile soil extracts salts

medium solution. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Amphora roettgeri.

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Soil Seawater Medium for Algae