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Sonneborn's Paramecium Medium

[所属分类:培养基配方] [发布时间:2021-8-18] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1......................................................................................1.0L

Klebsiella pneumoniae cultured on solution 2........................ variable

Solution 1:

Composition per liter:

Rye grass cerophyll....................................................................... 2.5g

Na2HPO4 ....................................................................................... 0.5g

Preparation of Solution 1: Add cerophyll to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Boil for 5 min. Filter through Whatman #1 filter paper. Add

0.5g of Na2HPO4. Bring volume to 1.0L with distilled/deionized water.

Mix thoroughly. Distribute 10.0mL volumes into tubes. Autoclave for

15 min at 15 psi pressure–121°C.

Source: Cerophyll can be obtained from Ward’s Natural Science Es

tablishment, Inc. Dairy Goat Nutrition distributes Grass Media Cul

ture, which is equivalent. Cereal Leaf Product from Sigma Chemical is

similar to cerophyll.

Solution 2:

Composition per liter:

Agar ............................................................................................ 20.0g

Yeast extract.................................................................................. 4.0g

Glucose ....................................................................................... 0.16g

Preparation of Solution 2: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute 5.0mL volumes into tubes. Autoclave for 15 min

at 15 psi pressure–121°C. Allow tubes to cool in a slanted position.

Preparation of Medium: Inoculate the surface of the agar slants of

solution 2 with a culture of Klebsiella pneumoniae. Incubate at 37°C

for 24–48 hr. Scrape cells from the surface of the agar slants and add to

10.0mL of solution 1. Incubate at 30°C for 24 hr. Inoculate with proto

zoa.

Use: For the cultivation of a variety of Paramecium species, Bodo spe

cies, Cercomonas species, Chlamydophrys species, Cyclidium species,

Dimastigella species, Euplotes aediculatus, Glaucoma chattoni, Recli

nomonas americana, Rhabdostyla species, Rhynchomonas nasuta,

Stachyamoeba species, Thaumatomastix species, and Trichamoeba

species.