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Sorangium Medium

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

Sorangium Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 10.0g

KNO3 ............................................................................................ 1.0g

K2HPO4......................................................................................... 1.0g

MgSO4 .......................................................................................... 0.2g

CaCl2............................................................................................. 0.1g

FeCl3 ........................................................................................... 0.02g

Preparation of Medium: Add components to tap water and bring

volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Pour into sterile Petri dishes or leave in tubes. Allow tubes to

cool in a slanted position. Aseptically add a sterile strip (4.5cm ×

1.0cm) of Whatman #1 filter paper to the surface of each slant or 4–6

sterile strips of filter paper to the surface of each agar plate.

Use: For the cultivation and maintenance of Polyangium cellulosum.