培养基配方

您现在的位置：网站首页 >> 培养基配方

Sorbitol Agar

[所属分类:培养基配方] [发布时间:2021-8-19] [发布人:网站管理员2] [阅读次数:] [返回]

Sorbitol Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Peptone ....................................................................................... 10.0g

NaCl.............................................................................................. 2.0g

Yeast extract.................................................................................. 2.0g

Sorbitol solution.......................................................................50.0mL

pH 7.0 ± 0.2 at 25°C

Sorbitol Solution:

Composition per 50.0mL:

Sorbitol ......................................................................................... 5.0g

Preparation of Sorbitol Solution: Add sorbitol to distilled/deion

ized water and bring volume to 50.0mL. Mix thoroughly. Filter steril

ize.

Preparation of Medium: Add components, except sorbitol solu

tion, to distilled/deionized water and bring volume to 950.0mL. Mix

thoroughly. Adjust pH to 7.0. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add sterile sorbitol solution. Mix thoroughly. Pour into sterile

Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of Pseudomonas species.