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Standard Methods Caseinate Agar

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

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Composition per liter:

Agar ............................................................................................ 15.0g

Sodium caseinate ........................................................................ 10.0g

Pancreatic digest of casein............................................................ 5.0g

Yeast extract.................................................................................. 2.5g

Glucose ......................................................................................... 1.0g

Trisodium citrate solution.............................................................1.0L

CaCl2·2H2O (1M solution).......................................................20.0mL

Trisodium Citrate Solution:

Composition per liter:

Trisodium citrate·2H2O............................................................... 4.41g

Preparation of Trisodium Citrate Solution: Add trisodium

citrate·2H2O to distilled/deionized water and bring volume to 1.0L.

Mix thoroughly.

Preparation of Medium: Add yeast extract, pancreatic digest of ca

sein, glucose, and agar to 500.0mL of the trisodium citrate solution. Mix

thoroughly. Gently heat until boiling. Add the remaining components.

Bring volume to 1.0L with trisodium citrate solution. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into sterile Petri

dishes.

Use: For the cultivation of proteolytic bacteria from foods.