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Standard Methods Caseinate HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Sodium caseinate ........................................................................ 10.0g

Plant hydrolysate .......................................................................... 5.0g

Na3-citrate................................................................................... 4.41g

Yeast extract.................................................................................. 2.5g

CaCl2........................................................................................... 2.22g

Glucose ......................................................................................... 1.0g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Pour into sterile Petri dishes in 20.0mL vol

umes. Allow the surface of the plates to dry before inoculation.

Use: For the cultivation of proteolytic bacteria from foods.