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Stanier’s Basal Medium with Pyridoxine and Yeast Extract

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

Stanier’s Basal Medium

with Pyridoxine and Yeast Extract

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4....................................................................................... 2.78g

Na2HPO4..................................................................................... 2.78g

(NH4)2SO4 .................................................................................... 1.0g

Yeast extract.................................................................................. 0.2g

Hutner’s mineral base ..............................................................20.0mL

Pyridoxine solution..................................................................10.0mL

pH 6.8 ± 0.2 at 25°C

Pyridoxine Solution:

Composition per 10.0mL:

Pyridoxine..................................................................................... 2.0g

Preparation of Pyridoxine Solution: Add pyridoxine to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Filter

sterilize.

Hutner’s Mineral Base:

Composition per liter:

MgSO4·7H2O.............................................................................. 29.7g

Nitrilotriacetic acid ..................................................................... 10.0g

CaCl2·2H2O ................................................................................ 3.34g

FeSO4·7H2O............................................................................. 99.0mg

(NH4)2MoO4 ............................................................................ 9.25mg

Metals “44”..............................................................................50.0mL

Preparation of Hutner’s Mineral Base: Add nitrilotriacetic acid

to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to 6.5

with KOH. Add remaining components. Readjust pH to 7.2 with H2SO4 or

KOH. Add distilled/deionized water to 1.0L.

Metals “44”:

Composition per 100.0mL:

ZnSO4·7H2O ................................................................................. 1.1g

FeSO4·7H2O.................................................................................. 0.5g

EDTA .......................................................................................... 0.25g

MnSO4·7H2O ............................................................................ 0.154g

CuSO4·5H2O ............................................................................... 0.04g

Co(NO3)2·6H2O ........................................................................ 0.025g

Na2B4O7·10H2O........................................................................ 0.018g

Preparation of Metals “44”: Add a few drops of H2SO4 to dis

tilled/deionized water to inhibit precipitate formation. Add compo

nents to acidified distilled/deionized water and bring volume to

100.0mL. Mix thoroughly.

Preparation of Medium: Add components, except pyridoxine so

lution, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Adjust pH to 6.8 with 1N KOH. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile pyri

doxine solution. Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation and maintenance of Pseudomonas species.

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Stanier’s Basal Medium with Pyridoxine and Yeast Extract