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Stanier’s Basal Medium with Trichlorophenoxyacetate

[所属分类:培养基配方] [发布时间:2021-8-25] [发布人:网站管理员2] [阅读次数:] [返回]

Stanier’s Basal Medium

with Trichlorophenoxyacetate

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

KH2PO4....................................................................................... 2.78g

Na2HPO4 ..................................................................................... 2.78g

(NH4)2SO4..................................................................................... 1.0g

2,4,5-Trichlorophenoxyacetate ..................................................... 1.0g

Hutner’s mineral base ..............................................................20.0mL

Hutner’s Mineral Base:

Composition per liter:

MgSO4·7H2O .............................................................................. 29.7g

Nitrilotriacetic acid ..................................................................... 10.0g

CaCl2·2H2O................................................................................. 3.34g

FeSO4·7H2O.................................................................................. 0.1g

(NH4)2MoO4 ............................................................................9.25mg

Metals “44” ..............................................................................50.0mL

Preparation of Hutner’s Mineral Base: Add nitrilotriacetic acid

to 500.0mL of distilled/deionized water. Dissolve by adjusting pH to

6.5 with KOH. Add remaining components. Readjust pH to 7.2 with

H2SO4 or KOH. Add distilled/deionized water to 1.0L. Store at 5°C.

Metals “44”:

Composition per 100.0mL:

ZnSO4·7H2O ................................................................................. 1.1g

FeSO4·7H2O.................................................................................. 0.5g

EDTA .......................................................................................... 0.25g

MnSO4·7H2O ............................................................................ 0.154g

CuSO4·5H2O ............................................................................... 0.04g

Co(NO3)2·6H2O ........................................................................ 0.025g

Na2B4O7·10H2O........................................................................ 0.018g

Preparation of Metals “44”: Add a few drops of H2SO4 to dis

tilled/deionized water to inhibit precipitate formation. Add compo

nents to acidified distilled/deionized water and bring volume to

100.0mL. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes

or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Pseudomonas cepacia.