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Starch Agar

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

Starch Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 25.0g

Starch, soluble............................................................................... 2.0g

Mineral solution.....................................................................200.0mL

Mineral Solution:

Composition per liter:

KH2PO4 ........................................................................................ 2.0g

(NH4)2SO4 .................................................................................... 1.4g

MgSO4·7H2O................................................................................ 0.3g

Calcium chloride solution........................................................10.0mL

Trace elements solution ...........................................................10.0mL

Calcium Chloride Solution:

Composition per 100.0mL:

CaCl2·2H2O .................................................................................. 3.0g

Preparation of Calcium Chloride Solution: Add CaCl2·2H2O

to distilled/deionized water and bring volume to 100.0mL. Mix thor

oughly.

Trace Elements Solution:

Composition per liter:

FeSO4·7H2O ................................................................................. 0.5g

CoCl2 ............................................................................................ 0.2g

MnSO4·H2O................................................................................ 0.16g

ZnSO4·7H2O............................................................................... 0.14g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Mineral Solution: Add components to distilled/

deionized water and bring volume to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Aspergillus awamori.