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Starch Agar with Bromcresol Purple

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Cornstarch................................................................................... 10.0g

Meat peptone............................................................................... 10.0g

Bromcresol Purple solution .......................................................1.2mL

pH 6.8 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ...................................................................... 0.16g

Ethanol (95% solution) ............................................................10.0mL

Preparation of Bromcresol Purple Solution: Add Bromcresol

Purple to 10.0mL of 95% ethanol. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the differentiation of Gardnerella vaginalis (Haemophilus

vaginalis, Corynebacterium vaginale) from other microorganisms

found in the genitourinary tract, with the exception of some strains of

Streptococcus and Lactobacillus. Differentiation is based on starch

hydrolysis. Bacteria that can hydrolyze starch appear as colonies sur

rounded by a yellow zone.