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Starch Agar with Bromcresol Purple

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1...............................................................................200.0mL

Solution 2.................................................................................20.0mL

pH 7.8 ± 0.2 at 25°C

Solution 1:

Composition per 200.0mL:

Heart infusion agar ....................................................................5.0mL

Bromcresol Purple solution .......................................................0.2mL

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 200.0mL. Mix thoroughly. Gently heat

while stirring and bring to boiling.

Heart Infusion Agar:

Composition per liter:

Beef heart, solids from infusion................................................ 500.0g

Agar ............................................................................................ 15.0g

Tryptose ...................................................................................... 10.0g

NaCl.............................................................................................. 5.0g

Preparation of Heart Infusion Agar: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gen

tly heat and bring to boiling.

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ...................................................................... 0.16g

Ethanol (95% solution)............................................................10.0mL

Preparation of Bromcresol Purple Solution: Add Bromcresol

Purple to 10.0mL of ethanol. Mix thoroughly.

Solution 2:

Composition per 20.0mL:

Starch ............................................................................................ 0.4g

Preparation of Solution 2: Add starch to distilled/deionized water

and bring volume to 20.0mL. Mix thoroughly. Gently heat while stir

ring and bring to boiling.

Preparation of Medium: Combine solution 1 and solution 2. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the differentiation of Gardnerella vaginalis (Haemophilus

vaginalis, Corynebacterium vaginale) from other microorganisms

found in the genitourinary tract, with the exception of some strains of

Streptococcus and Lactobacillus. Differentiation is based on starch

hydrolysis. Bacteria that can hydrolyze starch appear as colonies sur

rounded by a yellow zone.

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Starch Agar with Bromcresol Purple