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Starch Fermentation Broth

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 225.2mL:

Starch solution .........................................................................20.0mL

Heart infusion broth ...................................................................5.0mL

Bromcresol Purple solution .......................................................0.2mL

pH 7.8 ± 0.2 at 25°C

Heart Infusion Broth:

Composition per liter:

Beef heart, infusion from .......................................................... 500.0g

Tryptose ...................................................................................... 10.0g

NaCl .............................................................................................. 5.0g

pH 7.4 ± 0.2 at 25°C

Source: Heart infusion broth is available as a premixed powder from

BD Diagnostic Systems.

Preparation of Heart Infusion Broth: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Bromcresol Purple Solution:

Composition per 10.0mL:

Bromcresol Purple ........................................................................ 0.1g

Ethanol (95% solution) ............................................................10.0mL

Preparation of Bromcresol Purple Solution: Add Bromcresol

Purple to 10.0mL ethanol. Mix thoroughly.

Starch Solution:

Composition per 20.0mL:

Starch ............................................................................................ 0.4g

Preparation of Starch Solution: Add starch to distilled/deionized

water and bring volume to 20.0mL. Mix thoroughly. Gently heat while

stirring and bring to boiling.

Preparation of Medium: Combine 5.0mL of heart infusion broth,

0.2mL of Bromcresol Purple solution, 200.0mL of distilled/deionized

water, and 20.0mL of starch solution. Mix thoroughly. Distribute into

tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes or leave in tubes.

Use: For the cultivation of Corynebacterium species.