培养基配方

您现在的位置：网站首页 >> 培养基配方

Starch HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-8-26] [发布人:网站管理员2] [阅读次数:] [返回]

Starch HiVeg Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

Plant peptone ................................................................................ 5.0g

NaCl.............................................................................................. 5.0g

Starch, soluble............................................................................... 2.0g

Yeast extract.................................................................................. 1.5g

Plant extract .................................................................................. 1.5g

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and differentiation of a variety of microorgan

isms based on amylase production. After incubation, starch hydrolysis

is determined by the addition of Gram’s or Lugol’s iodine solution.

Organisms that produce amylase appear as colonies surrounded by a

clear zone.