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Sulfolobus acidocaldarius Simplified Basal Medium

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfolobus acidocaldarius

Simplified Basal Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

K2SO4 ........................................................................................... 6.0g

NaH2PO4....................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.6g

CaCl2·7H2O .................................................................................. 0.2g

Trace minerals solution............................................................0.04mL

pH 3.5 ± 0.2 at 25°C

Trace Minerals Solution:

Composition per 100.0mL:

FeCl3·6H2O................................................................................... 5.0g

CuCl2·2H2O .................................................................................. 0.5g

CoCl2·6H2O .................................................................................. 0.5g

MnCl2·2H2O ................................................................................. 0.5g

ZnCl2............................................................................................. 0.5g

HCl (1N solution) ................................................................... 100.0ml

Preparation of Trace Minerals Solution: Combine components.

Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.5 with

H2SO4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi

pressure–121°C.

Use: For the cultivation of Sulfolobus acidocaldarius.