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Sulfolobus brierleyi Medium

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur flowers ............................................................................. 10.0g

(NH4)2SO4 .................................................................................... 3.0g

K2HPO4·3H2O.............................................................................. 0.5g

MgSO4·7H2O................................................................................ 0.5g

KCl................................................................................................ 0.1g

Ca(NO3)...................................................................................... 0.01g

Yeast extract solution...............................................................20.0mL

pH 1.5–2.5 at 25°C

Preparation of Sulfur: Autoclave sulfur at 8 psi pressure–112°C

for 15 min.

Yeast Extract Solution:

Composition per 20.0mL:

Yeast extract.................................................................................. 0.2g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 20.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components, except yeast extract so

lution and sulfur, to distilled/deionized water and bring volume to

980.0mL. Mix thoroughly. Adjust pH with 6N H2SO4 to 1.5–2.5. Au

toclave for 15 min at 15 psi pressure–121°C. Aseptically add 20.0mL

of sterile yeast extract solution and 10.0g of sterile sulfur. Mix thor

oughly. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation of Acidianus brierleyi.