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Sulfolobus Broth

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfolobus Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose yeast solution............................................................500.0mL

CaCl2·2H2O solution..............................................................250.0mL

Trace elements solution .........................................................250.0mL

pH 3.0–3.5 at 25°C

Sucrose Yeast Solution:

Composition per 500.0mL:

Sucrose.......................................................................................... 2.0g

Yeast extract.................................................................................. 1.0g

Preparation of Sucrose Yeast Solution: Add components to dis

tilled/deionized water and bring volume to 500.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

CaCl2·2H2O Solution:

Composition per 250.0mL:

CaCl2·2H2O................................................................................... 2.0g

Preparation of CaCl2·2H2O Solution: Add CaCl2·2H2O to dis

tilled/deionized water and bring volume to 250.0mL. Mix thoroughly.

Autoclave for 15 min at 15 psi pressure–121°C.

Trace Elements Solution:

Composition per 250.0mL:

(NH4)2SO4..................................................................................... 1.3g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O .............................................................................. 0.25g

FeSO4·7H2O.............................................................................28.0mg

Na2B4O7·10H2O.........................................................................4.5mg

MnCl2·7H2O...............................................................................1.8mg

ZnSO4·7H2O ............................................................................0.22mg

CuCl2·2H2O .............................................................................0.05mg

NaMoO4·2H2O.........................................................................0.03mg

VOSO4·2H2O...........................................................................0.03mg

CoSO4·2H2O ............................................................................0.01mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 250.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 500.0mL of sterile

sucrose yeast solution with 250.0mL of sterile CaCl2·2H2O solution

and 250.0mL of sterile trace elements solution. Mix thoroughly. Adjust

pH to 3.0–3.5 with sterile H2SO4. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation of Sulfolobus species.