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Sulfolobus Medium

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfolobus Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Gellan sucrose yeast solution.................................................500.0mL

CaCl2·2H2O/MgCl2·6H2O solution........................................250.0mL

Trace elements solution .........................................................250.0mL

pH 3.0–3.5 at 25°C

Gellan Sucrose Yeast Solution:

Composition per 500.0mL:

Gellan gum.................................................................................... 6.5g

Sucrose.......................................................................................... 2.0g

Yeast extract.................................................................................. 1.0g

Source: Gellan gum is available from Kelco.

Preparation of Gellan Sucrose Yeast Solution: Add compo

nents to distilled/deionized water and bring volume to 500.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 60°C.

CaCl2·2H2O/MgCl2·6H2O Solution:

Composition per 250.0mL:

CaCl2·2H2O ................................................................................ 2.44g

MgCl2·6H2O ................................................................................. 2.0g

Preparation of CaCl2·2H2O/MgCl2·6H2O Solution: Add com

ponents to distilled/deionized water and bring volume to 250.0mL.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 60°C.

Trace Elements Solution:

Composition per 250.0mL:

(NH4)2SO4 .................................................................................... 1.3g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O.............................................................................. 0.25g

FeSO4·7H2O............................................................................. 28.0mg

Na2B4O7·10H2O......................................................................... 4.5mg

ZnSO4·7H2O ............................................................................ 0.22mg

CuCl2·2H2O ............................................................................. 0.05mg

NaMoO4·2H2O......................................................................... 0.03mg

VOSO4·2H2O........................................................................... 0.03mg

CoSO4·2H2O............................................................................ 0.01mg

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 250.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 60°C.

Preparation of Medium: Aseptically combine 500.0mL of sterile

gellan sucrose yeast solution with 250.0mL of sterile CaCl2·2H2O/

MgCl2·6H2O solution and 250.0mL of sterile trace elements solution.

Mix thoroughly. Adjust pH to 3.0–3.5 with sterile H2SO4. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Sulfolobus species.

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Sulfolobus Medium