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Sulfolobus Medium, Revised

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

(NH4)2SO4 .................................................................................... 1.3g

Tryptone........................................................................................ 1.0g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O.............................................................................. 0.25g

CaCl2·2H2O ................................................................................ 0.07g

Yeast extract................................................................................ 0.05g

FeCl3·6H2O................................................................................. 0.02g

Na2B4O7..................................................................................... 4.5mg

MnCl2·4H2O .............................................................................. 1.8mg

ZnSO4·7H2O ............................................................................ 0.22mg

CuCl2·H2O ...............................................................................0.05mg

Na2MoO4·H2O .........................................................................0.03mg

VOSO4·2H2O...........................................................................0.03mg

CoSO4 ......................................................................................0.01mg

pH 3.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH at 25°C to

3.0 with 10N H2SO4. Filter sterilize. Aseptically distribute into tubes or

flasks.

Use: For the cultivation and maintenance of Sulfolobus species.