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Sulfophobococcus zilligii Medium (DSMZ Medium 770)

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfophobococcus zilligii Medium

(DSMZ Medium 770)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1035.0mL:

Glycine.......................................................................................... 1.5g

Na2CO3 ..................................................................................230.0mg

CaCl2·2H2O ............................................................................. 66.0mg

Na2-EDTA................................................................................ 32.0mg

MgSO4·7H2O........................................................................... 31.0mg

KCl........................................................................................... 31.0mg

MnSO4·2H2O............................................................................. 2.3mg

ZnCl2.......................................................................................... 2.1mg

Na2B4O7·10H2O......................................................................... 1.8mg

Resazurin ................................................................................... 0.5mg

Serum albumin solution...........................................................10.0mL

Dithiothreitol solution..............................................................10.0mL

Yeast extract solution...............................................................10.0mL

Iron sulfate solution ...................................................................5.0mL

pH 7.6 ± 0.2 at 25°C

Dithiothreitol Solution:

Composition per 10.0mL:

Dithiothreitol........................................................................... 1.54mg

Preparation of Dithiothreitol Solution: Add dithiothreitol to

distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Iron Sulfate Solution:

Composition per 10.0mL:

FeSO4·7H2O................................................................................. 0.1g

Preparation of Iron Sulfate Solution: Add FeSO4·7H2O to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Serum Albumin Solution:

Composition per 10.0mL:

Bovine serum albumin, fraction V............................................... 1.0g

Preparation of Serum Albumin Solution: Add bovine serum al

bumin, fraction V to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Yeast Extract Solution:

Composition per 10.0mL:

Yeast extract.................................................................................. 1.0g

Preparation of Yeast Extract Solution: Add yeast extract to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Sparge with 100% N2. Autoclave under 100% N2 for 15 min at 15 psi

pressure–121°C. Cool to room temperature.

Preparation of Medium: Prepare medium anaerobically under

100% N2 gas. Add components, except iron sulfate solution, serum albu

min solution, dithiothreitol solution, and yeast extract solution, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Cool to 80–90°C. Adjust pH to 7.6. Cool to

room temperature. Dispense 30.0mL aliquots into serum bottles. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to room temperature.

Aseptically inject per each 30.0mL the following solutions: 0.3mL ster

ile yeast extract solution, 0.3mL sterile dithiothreitol solution, 0.15mL

sterile iron sulfate solution, and 10.0mL sterile serum albumin solution.

Final pH should be 7.6.

Use: For the cultivation of Sulfophobococcus zilligii.

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Sulfophobococcus zilligii Medium (DSMZ Medium 770)