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Sulforhabdus Medium (DSMZ Medium 386a)

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

Sulforhabdus Medium

(DSMZ Medium 386a)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1002.0mL:

Solution A..............................................................................920.0mL

Solution C ................................................................................50.0mL

Solution D................................................................................10.0mL

Solution E ................................................................................10.0mL

Solution F.................................................................................10.0mL

Solution B

(Trace elements solution SL-10B).......................................1.0mL

pH 7.2–7.5 at 25°C

Solution A:

Composition per 920.0mL:

NaCl .............................................................................................. 1.0g

KCl................................................................................................ 0.5g

MgCl2·6H2O.................................................................................. 0.4g

KH2PO4......................................................................................... 0.2g

NH4Cl ........................................................................................... 0.3g

CaCl2·2H2O................................................................................. 0.15g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 920.0mL. Mix thoroughly. Sparge with 80%

N2 + 20% CO2 gas until saturated. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 25°C.

Solution B (Trace Elements Solution SL-10B):

Composition per liter:

FeCl2·4H2O ................................................................................... 1.5g

H3BO3 ....................................................................................300.0mg

CoCl2·6H2O ...........................................................................190.0mg

MnCl2·4H2O...........................................................................100.0mg

ZnCl2........................................................................................70.0mg

Na2MoO4·2H2O .......................................................................36.0mg

NiCl2·6H2O ..............................................................................24.0mg

CuCl2·2H2O ...............................................................................2.0mg

HCl (25% solution)....................................................................7.7mL

Preparation of Solution B (Trace Elements Solution SL-10B):

Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add dis

tilled/deionized water and bring volume to 1.0L. Add remaining com

ponents. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min

at 15 psi pressure–121°C.

Solution C:

Composition per 100.0mL:

NaHCO3 ........................................................................................ 5.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly. Sparge with 80%

N2 + 20% CO2 gas until saturated, approximately 20 min. Filter steril

ize under 100% CO2 into a sterile, gas-tight 100.0mL screw-capped

bottle.

Solution D:

Composition per 10.0mL:

Na2-acetate·3H2O.......................................................................... 0.3g

Preparation of Solution D: Add Na2-acetate to distilled/deionized

water and bring volume to 10.0mL. Sparge with N2. Filter sterilize.

Store anaerobically.

Solution E:

Composition per 10.0mL:

Na2S·9H2O.................................................................................... 0.4g

Preparation of Solution E: Add Na2S·9H2O to distilled/deionized

water and bring volume to 10.0mL. Sparge with N2. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 25°C. Store anaerobically.

Solution F:

Composition per 10.0mL:

Na-thiosulfate................................................................................ 2.5g

Preparation of Solution F: Add Na-thiosulfate to distilled/deion

ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril

ize. Flush with 80% N2 + 20% CO2 to remove dissolved oxygen.

Preparation of Medium: Add solution B, solution C, solution D,

solution E, and solution F to solution A in that order under 80% N2 +

20% CO2 gas. Adjust the pH to 7.2–7.5.

Use: For the cultivation of Desulfocapsa thiozymogenes.

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Sulforhabdus Medium (DSMZ Medium 386a)