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培养基配方

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Sulfurospirillum Medium

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1004.0mL:

Solution A ..............................................................................900.0mL

Solution C ................................................................................80.0mL

Solution D ................................................................................20.0mL

Solution B (Trace elements solution SL-10) .............................2.0mL

Solution E ..................................................................................2.0mL

pH 7.2 ± 0.2 at 25°C

Solution A:

Composition per 900.0mL:

KH2PO4....................................................................................... 1.36g

MgSO4·7H2O.............................................................................. 0.37g

NH4Cl ......................................................................................... 0.27g

CaCl2·2H2O .................................................................................. 0.1g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Sparge with 80%

N2 + 20% CO2. Anaerobically distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C.

Solution B (Trace Elements Solution SL-10):

Composition per liter:

FeCl2·4H2O................................................................................... 1.5g

CoCl2·6H2O ........................................................................... 190.0mg

MnCl2·4H2O .......................................................................... 100.0mg

ZnCl2........................................................................................ 70.0mg

Na2MoO4·2H2O ....................................................................... 36.0mg

NiCl2·6H2O.............................................................................. 24.0mg

H3BO3 ........................................................................................ 6.0mg

CuCl2·2H2O ............................................................................... 2.0mg

HCl (25% solution)..................................................................10.0mL

Preparation of Solution B (Trace Elements Solution SL-10):

Add FeCl2·4H2O to 10.0mL of HCl solution. Mix thoroughly. Add dis

tilled/deionized water and bring volume to 1.0L. Add remaining com

ponents. Mix thoroughly. Sparge with 100% N2. Autoclave for 15 min

at 15 psi pressure–121°C.

Solution C:

Composition per 80.0mL:

NaHCO3........................................................................................ 4.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized

water and bring volume to 80.0mL. Mix thoroughly. Sparge with 80%

N2 + 20% CO2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution D:

Composition per 20.0mL:

Sodium fumarate........................................................................... 4.0g

Preparation of Solution D: Add sodium fumarate to distilled/de

ionized water and bring volume to 20.0mL. Mix thoroughly. Sparge

with 100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Solution E:

Composition per 2.0mL:

L-Cysteine·HCl ......................................................................... 0.063g

Preparation of Solution E: Add L-cysteine·HCl to distilled/deion

ized water and bring volume to 2.0mL. Mix thoroughly. Sparge with

100% N2. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: To sterile solution A in tubes or flasks,

add, using a syringe, appropriate volumes of sterile solution B, solution

C, solution D, and solution E. Mix thoroughly.

Use: For the cultivation of Sulfurospirillum deleyianum.

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Sulfurospirillum Medium