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Sulfurospirillum Medium

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................953.0mL

Solution B ................................................................................42.0mL

Solution C ..................................................................................5.0mL

pH 7.2 ± 0.2 at 25°C

Solution A:

Composition per 953.0mL:

KH2PO4....................................................................................... 1.36g

MgSO4·7H2O .............................................................................. 0.37g

NH4Cl ......................................................................................... 0.27g

CaCl2·2H2O................................................................................. 0.10g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 953.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to room temperature while sparg

ing with 90% N2 + 10% CO2.

Solution B:

Composition per 42.0mL:

Sodium fumarate........................................................................... 4.0g

NaHCO3 ........................................................................................ 2.0g

Trace elements solution SL-10 ..................................................2.0mL

Trace Elements Solution SL-10:

Composition per liter:

FeCl2·4H2O ................................................................................... 1.5g

CoCl2·6H2O ...........................................................................190.0mg

MnCl2·4H2O...........................................................................100.0mg

ZnCl2........................................................................................70.0mg

Na2MoO4·2H2O .......................................................................36.0mg

NiCl2·6H2O ..............................................................................24.0mg

H3BO3 ........................................................................................6.0mg

CuCl2·2H2O ...............................................................................2.0mg

HCl (25% solution)..................................................................10.0mL

Preparation of Trace Elements Solution SL-10: Add FeCl2·4H2O

to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized

water and bring volume to 1.0L. Add remaining components. Mix thor

oughly.

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 42.0mL. Mix thoroughly. Filter sterilize.

Solution C:

Composition per 5.0mL:

L-Cysteine·HCl.......................................................................... 0.063g

Preparation of Solution C: Add L-cysteine·HCl to distilled/deion

ized water and bring volume to 5.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Aseptically combine 953.0mL of sterile

solution A with 42.0mL of sterile solution B and 5.0mL of sterile solu

tion C. Prepare freshly. Adjust pH to 7.2 with sterile 2M Na2CO3 solu

tion or sterile 2N HCl solution.

Use: For the cultivation of Sulfurospirillum deleyianum.

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Sulfurospirillum Medium