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Supplemented Aspergillus Minimal Agar

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Glucose ....................................................................................... 20.0g

NaNO3 .......................................................................................... 6.0g

(NH4)2SO4 .................................................................................... 5.0g

Casamino acids ............................................................................. 2.0g

K2HPO4 ...................................................................................... 1.52g

KH2PO4......................................................................................... 1.0g

KCl.............................................................................................. 0.52g

MgSO4·7H2O.............................................................................. 0.52g

NaCl.............................................................................................. 0.1g

CaCl2·2H2O .................................................................................. 0.1g

Inositol ....................................................................................... 2.0mg

KI ............................................................................................... 1.0mg

H3BO3 ........................................................................................ 0.5mg

ZnSO4·7H2O .............................................................................. 0.4mg

MnSO4·4H2O............................................................................. 0.4mg

Thiamine·HCl ............................................................................ 0.4mg

Pyroxidine·HCl .......................................................................... 0.4mg

Niacin......................................................................................... 0.4mg

Calcium pantothenate ................................................................ 0.4mg

p-Aminobenzoic acid................................................................. 0.2mg

Riboflavin .................................................................................. 0.2mg

Na2MoO4·4H2O ......................................................................... 0.2mg

CuSO4·5H2O............................................................................ 0.04mg

Folic acid ....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

FeCl3·6H2O.................................................................................1.0μg

ZnSO4·7H2O...............................................................................1.0μg

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5.

Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-

clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the cultivation and maintenance of Aspergillus species.