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Supplemented Aspergillus Minimal Broth

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ....................................................................................... 20.0g

NaNO3 .......................................................................................... 6.0g

(NH4)2SO4..................................................................................... 5.0g

Casamino acids ............................................................................. 2.0g

K2HPO4 ...................................................................................... 1.52g

KH2PO4......................................................................................... 1.0g

KCl.............................................................................................. 0.52g

MgSO4·7H2O.............................................................................. 0.52g

NaCl .............................................................................................. 0.1g

CaCl2·2H2O................................................................................... 0.1g

Inositol .......................................................................................2.0mg

KI ...............................................................................................1.0mg

H3BO3 ........................................................................................0.5mg

ZnSO4·7H2O ..............................................................................0.4mg

MnSO4·4H2O .............................................................................0.4mg

Thiamine·HCl ............................................................................0.4mg

Pyroxidine·HCl ..........................................................................0.4mg

Niacin.........................................................................................0.4mg

Calcium pantothenate ................................................................0.4mg

p-Aminobenzoic acid.................................................................0.2mg

Riboflavin ..................................................................................0.2mg

Na2MoO4·4H2O .........................................................................0.2mg

CuSO4·5H2O ............................................................................0.04mg

Folic acid.....................................................................................2.0μg

Biotin ..........................................................................................2.0μg

FeCl3·6H2O.................................................................................1.0μg

ZnSO4·7H2O...............................................................................1.0μg

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Aspergillus species.