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SWMTY Marine Medium

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

SWMTY Marine Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Marine salts mix ......................................................................... 38.0g

Agar ............................................................................................ 15.0g

Pancreatic digest of casein............................................................ 2.0g

Yeast extract.................................................................................. 2.0g

Tris(hydroxymethyl)aminomethane buffer................................... 1.0g

KNO3 ............................................................................................ 0.5g

Sodium glycerophosphate............................................................. 0.1g

Trace elements solution HO-LE ................................................1.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution HO-LE:

Composition per liter:

H3BO3 ......................................................................................... 2.85g

MnCl2·4H2O ................................................................................. 1.8g

Sodium tartrate............................................................................ 1.77g

FeSO4·7H2O................................................................................ 1.36g

CoCl2·6H2O ................................................................................ 0.04g

CuCl2·2H2O............................................................................... 0.027g

Na2MoO4·2H2O ........................................................................ 0.025g

ZnCl2........................................................................................... 0.02g

Preparation of Trace Elements Solution HO-LE: Add compo

nents to distilled/deionized water and bring volume to 1.0L. Mix thor

oughly. Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of a variety of heterotrophic

marine bacterial species.