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SXT Blood Agar

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

SXT Blood Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein.......................................................... 14.5g

Agar ............................................................................................ 14.0g

NaCl .............................................................................................. 5.0g

Papaic digest of soybean meal ...................................................... 5.0g

Growth factor, BBL ...................................................................... 1.5g

Sulfamethoxazole...................................................................... 0.024g

Trimethoprim ...........................................................................1.25mg

Sheep blood, defibrinated ........................................................50.0mL

pH 7.3 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components, except defibrinated

sheep blood, to distilled/deionized water and bring volume to

950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 50.0mL of defibrinated sheep blood. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the selective isolation of Lancefield group A and group B

streptococci from throat cultures and other clinical specimens.