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SY Broth

[所属分类:培养基配方] [发布时间:2021-8-31] [发布人:网站管理员2] [阅读次数:] [返回]

SY Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

(NH4)2SO4..................................................................................... 2.0g

Na2HPO4·2H2O............................................................................. 1.4g

KH2PO4......................................................................................... 0.7g

MgSO4·7H2O ................................................................................ 0.2g

FeSO4 .........................................................................................5.0mg

MnSO4 .......................................................................................5.0mg

Glucose solution ....................................................................100.0mL

Glucose Solution:

Composition per 100.0mL:

D-Glucose .................................................................................... 10.0g

Preparation of Glucose Solution: Add D-glucose to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Add components, except glucose solu

tion, to distilled/deionized water and bring volume to 900.0mL. Mix

thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile glu

cose solution. Mix thoroughly. Aseptically distribute into sterile tubes

or flasks.

Use: For the isolation and cultivation of Cytophaga species, Herpeto

siphon species, Saprospira species, and Flexithrix species.