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培养基配方
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Synthetic Complete Medium
[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]
Synthetic Complete Medium
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Solution A..............................................................................600.0mL
Solution B ..............................................................................400.0mL
Solution A:
Composition per 600.0mL:
Agar ............................................................................................ 20.0g
Preparation of Solution A: Add agar to distilled/deionized water
and bring volume to 600.0mL. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45–
50°C.
Solution B:
Composition per 400.0mL:
Glucose ....................................................................................... 20.0g
Yeast nitrogen base without amino acids...................................... 6.7g
L-Leucine .................................................................................... 0.18g
L-Alanine ................................................................................. 90.0mg
L-Arginine................................................................................ 90.0mg
L-Asparagine............................................................................ 90.0mg
L-Aspartic acid......................................................................... 90.0mg
L-Cysteine ................................................................................ 90.0mg
L-Glutamine ............................................................................. 90.0mg
L-Glutamic acid ....................................................................... 90.0mg
Glycine..................................................................................... 90.0mg
L-Histidine ............................................................................... 90.0mg
i-Inositol................................................................................... 90.0mg
L-Isoleucine.............................................................................. 90.0mg
L-Lysine ................................................................................... 90.0mg
L-Methionine............................................................................ 90.0mg
L-Phenylalanine ....................................................................... 90.0mg
L-Proline .................................................................................. 90.0mg
L-Serine.................................................................................... 90.0mg
L-Threonine.............................................................................. 90.0mg
L-Tryptophan ........................................................................... 90.0mg
L-Tyrosine ................................................................................ 90.0mg
L-Valine.................................................................................... 90.0mg
Adenine.................................................................................... 23.0mg
p-Aminobenzoic acid................................................................. 9.0mg
Preparation of Solution B: Add components to distilled/deionized
water and bring volume to 400.0mL. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C prior to preparation of medium.
Preparation of Medium: Aseptically combine 600.0mL of sterile
solution A with 400.0mL of sterile solution B. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Saccharomyces cerevi
siae.
