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Synthetic Malate Medium with 0.25% Sodium Chloride

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

Synthetic Malate Medium

with 0.25% Sodium Chloride

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

DL-Malic acid................................................................................ 5.0g

KOH.............................................................................................. 4.5g

NaCl .............................................................................................. 2.5g

KH2PO4......................................................................................... 0.6g

NH4Cl ........................................................................................... 0.5g

K2HPO4......................................................................................... 0.4g

MgSO4·7H2O ................................................................................ 0.2g

Yeast extract.................................................................................. 0.1g

CaCl2........................................................................................... 0.02g

MnSO4·H2O ................................................................................ 0.01g

Na2MoO4·2H2O ........................................................................ 0.002g

Biotin .........................................................................................0.1mg

Ferric EDTA solution...............................................................10.0mL

pH 7.2 ± 0.2 at 25°C

Ferric EDTA Solution:

Composition per 100.0mL:

Ferric EDTA................................................................................ 0.66g

Preparation of Ferric EDTA Solution: Add ferric EDTA to dis

tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.

Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C.

Use: For the cultivation of Azospirillum halopraeferens.