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SYPC Medium (DSMZ Medium 1188)

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

SYPC Medium

(DSMZ Medium 1188)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sea salts, Sigma .......................................................................... 35.0g

Yeast extract ................................................................................. 1.0g

Trypticase polypeptone................................................................. 0.5g

Lactate solution........................................................................10.0mL

Wolfe's mineral elixir.................................................................1.0mL

pH 7.5 ± 0.2 at 25°C

Cellobiose Solution:

Composition per 10.0mL:

Cellobiose ..................................................................................... 2.0g

Preparation of Cellobiose Solution: Add cellobiose to distilled/

deionized water and bring volume to 10.0mL. Mix thoroughly. Sparge

with 100% N2. Filter sterilize.

Wolfe’s Mineral Elixir:

Composition per liter:

MgSO4·7H2O .............................................................................. 30.0g

NaCl ............................................................................................ 10.0g

MnSO4·2H2O ................................................................................ 5.0g

(NH4)2NiSO4·6H2O ...................................................................... 2.8g

CoCl2·6H2O .................................................................................. 1.8g

ZnSO4·7H2O ................................................................................. 1.8g

FeSO4·7H2O.................................................................................. 1.0g

CaCl2·2H2O................................................................................... 1.0g

KAl(SO4)2·12H2O....................................................................... 0.18g

CuSO4·5H2O ................................................................................. 0.1g

H3BO3 ........................................................................................... 0.1g

Na2MoO4·2H2O ............................................................................ 0.1g

Na2SeO4 ........................................................................................ 0.1g

Na2WO4·2H2O .............................................................................. 0.1g