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T 7Agar Base (m-T7 Agar Base)

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

T 7Agar Base

(m-T7 Agar Base)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose ........................................................................................ 20.0g

Agar ............................................................................................ 15.0g

Polyoxyethylene ether W-1........................................................... 5.0g

Yeast extract.................................................................................. 3.0g

Pancreatic digest of casein............................................................ 2.5g

Peptic digest of animal tissue ....................................................... 2.5g

Sodium heptadecyl sulfate............................................................ 0.1g

Bromthymol Blue ......................................................................... 0.1g

Bromcresol Purple ........................................................................ 0.1g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat while

stirring and bring to boiling. Distribute into tubes or flasks. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. The medium

may be made more selective by adding 1.0mg of penicillin G per liter.

Pour into sterile Petri dishes or leave in tubes.

Use: For the selective recovery and differential identification of

injured coliform microorganisms from chlorinated water by the mem

brane filter method. For rapid estimation of the bacteriological quality

of water using the membrane filter method.