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T-ASW Medium

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

T-ASW Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1003.0mL:

NaCl............................................................................................ 25.0g

Na2S2O3·5H2O .............................................................................. 2.5g

MgSO4·7H2O................................................................................ 1.5g

(NH4)2SO4 .................................................................................... 1.0g

KH2PO4......................................................................................... 0.4g

CaCl2·2H2O .................................................................................. 0.3g

NaHCO3........................................................................................ 0.2g

Tris·HCl buffer, 0.1M, pH 7.5................................................200.0mL

Phenol Red (0.5% solution).......................................................2.0mL

Trace elements solution .............................................................1.0mL

pH 7.5 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

Disodium EDTA ......................................................................... 50.0g

CaCl2·2H2O .................................................................................. 5.5g

MnCl2·4H2O ................................................................................. 5.1g

FeSO4·7H2O.................................................................................. 5.0g

ZnSO4·7H2O ................................................................................. 2.2g

CoCl2·6H2O .................................................................................. 1.6g

CuSO4·5H2O................................................................................. 1.6g

(NH4)6Mo7O24·4H2O .................................................................... 1.1g

Preparation of Trace Elements Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust

pH to 6.0 with KOH. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.5. Fil

ter sterilize.

Use: For the cultivation and maintenance of Thiobacillus hydrotherma

lis.