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T2 Medium for Thiobacillus

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution A..............................................................................250.0mL

Solution B ..............................................................................250.0mL

Solution C ..............................................................................250.0mL

Solution D..............................................................................250.0mL

pH 7.0 ± 0.2 at 25°C

Solution A:

Composition per 250.0mL:

Na2S2O3·5H2O .............................................................................. 5.0g

KNO3 ............................................................................................ 2.0g

NH4Cl ........................................................................................... 1.0g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.

Solution B:

Composition per 250.0mL

KH2PO4......................................................................................... 2.0g

Preparation of Solution B: Add KH2PO4 to distilled/deionized

water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.

Solution C:

Composition per 250.0mL

NaHCO3 ........................................................................................ 2.0g

Preparation of Solution C: Add NaHCO3 to distilled/deionized

water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.

Solution D:

Composition per 250.0mL

MgSO4·7H2O ................................................................................ 0.8g

FeSO4·7H2O (2%, w/v, in 1N HCl)............................................1.0mL

Trace metal solution...................................................................1.0mL

Preparation of Solution D: Add components to distilled/deionized

water and bring volume to 250.0mL. Mix thoroughly. Filter sterilize.

FeSO4·7H2O Solution:

Composition per 100.0mL

FeSO4·7H2O.................................................................................. 2.0g

HCl (1N solution)...................................................................100.0mL

Preparation of FeSO4·7H2O Solution: Add the FeSO4·7H2O to

the HCl solution. Mix thoroughly.

Trace Metals Solution:

Composition per liter:

EDTA .......................................................................................... 50.0g

ZnSO4 ......................................................................................... 22.0g

CaCl2........................................................................................... 5.54g

MnCl2.......................................................................................... 5.06g

FeSO4·7H2O................................................................................ 4.99g

CoCl2........................................................................................... 1.61g

CuSO4 ......................................................................................... 1.57g

(NH4)2MoO4 ................................................................................. 1.1g

Preparation of Trace Metals Solution: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Ad

just pH to 6.0 with KOH.

Preparation of Medium: Aseptically combine the four sterile solu

tions: solution A, solution B, solution C, and solution D. Adjust the pH

to 7.0. Aseptically distribute into sterile tubes or flasks.

Use: For the cultivation and maintenance of Thiobacillus denitrificans

and other thiobacilli.

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T2 Medium for Thiobacillus