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TB Broth Base

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

TB Broth Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone ........................................................................... 4.0g

Na2HPO4 ....................................................................................... 2.5g

Yeast extract.................................................................................. 2.0g

Sodium citrate ............................................................................... 1.5g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O ................................................................................ 0.6g

Polysorbate 80............................................................................... 0.5g

Bovine albumin solution..........................................................50.0mL

Glucose solution ......................................................................10.0mL

Glycerol ....................................................................................5.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available from HiMedia.

Glucose Solution:

Composition per 10.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril

ize.

Bovine Albumin Solution:

Composition per 50.0mL:

Bovine serum albumin .................................................................. 5.0g

Preparation of Bovine Albumin Solution: Add bovine serum al

buin to distilled/deionized water and bring volume to 50.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add components, except bovine albumin

and glucose solution, to distilled/deionized water and bring volume to

960.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 50°C. Aseptically add glucose solution and bovine al

bumin solution. Mix thoroughly. Aseptically distribute into sterile

tubes or flasks.

Use: For the cultivation of Mycobacterium tuberculosis.