培养基配方

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TB Broth Base without Polysorbate 80

[所属分类:培养基配方] [发布时间:2021-9-1] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone........................................................................... 4.0g

Na2HPO4....................................................................................... 2.5g

Yeast extract.................................................................................. 2.0g

Sodium citrate............................................................................... 1.5g

KH2PO4......................................................................................... 1.0g

MgSO4·7H2O................................................................................ 0.6g

Bovine albumin solution..........................................................50.0mL

Glucose solution ......................................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available from HiMedia.

Glucose Solution:

Composition per 10.0mL:

Glucose ......................................................................................... 5.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 10.0mL. Mix thoroughly. Filter steril

ize.

Bovine Albumin Solution:

Composition per 50.0mL:

Bovine serum albumin.................................................................. 5.0g

Preparation of Bovine Albumin Solution: Add bovine serum al

bumin to distilled/deionized water and bring volume to 50.0mL. Mix

thoroughly. Filter sterilize.

Preparation of Medium: Add glycerol to distilled/deionized water

and bring volume to 955.0mL. Mix thoroughly. Add remaining com

ponents, except bovine albumin and glucose solutions, Autoclave for

15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add glu

cose solution and bovine albumin solution. Mix thoroughly. Aseptical

ly distribute into sterile tubes or flasks.

Use: For the cultivation of Mycobacterium tuberculosis.