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TB HiVeg Broth Base without Tween™ 80 with Bovine Serum and Glucose

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

TB HiVeg Broth Base without Tween™ 80

with Bovine Serum and Glucose

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Plant peptone No. 3....................................................................... 4.0g

Na2HPO4....................................................................................... 2.5g

Yeast extract.................................................................................. 2.0g

Sodium citrate............................................................................... 1.5g

KH2PO4......................................................................................... 1.0g

MgSO4 .......................................................................................... 0.6g

Glucose solution ......................................................................50.0mL

Bovine serum...........................................................................50.0mL

pH 7.0 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Glucose Solution:

Composition per 100.0mL:

Glucose ....................................................................................... 10.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Preparation of Medium: Add components, except bovine serum

and glucose solution, to distilled/deionized water and bring volume to

900.0L. Mix thoroughly. Gently heat until boiling. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C. Add 50.0mL sterile glu

cose solution and 50.0mL bovine serum. Mix thoroughly. Aseptically

distribute into sterile tubes.

Use: For the cultivation of mycobacteria when the presence of oleic

acid is undesirable. For the cultivation of Mycobacterium tuberculosis.