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TB Nitrate Reduction Broth

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100.0mL:

Na2HPO4·12H2O....................................................................... 0.485g

KH2PO4..................................................................................... 0.117g

NaNO3 ...................................................................................... 0.085g

pH 7.0 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the differentiation of Mycobacterium species based on nitrate

reduction. After growth of cells in appropriate medium, nitrate reduc

tion is determined by making a suspension of cells in TB nitrate reduc

tion broth and adding hydrochloric acid, sulfanilamide, and N-naphyl

enendiamine. Nitrate reduction turns the medium pink. Mycobacterium

tuberculosis reduces nitrate and turns the medium deep pink within 1

min. Mycobacterium bovis does not reduce nitrate and does not change

the medium.