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TBAB 298 Medium (Tryptose 298 Blood Agar Base Medium)

[所属分类:培养基配方] [发布时间:2021-9-2] [发布人:网站管理员2] [阅读次数:] [返回]

TBAB 298 Medium

(Tryptose 298 Blood Agar Base Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Tryptose Blood Agar Base:

Composition per 409.6mL:

Agar ............................................................................................ 15.0g

Tryptose ...................................................................................... 10.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

Glucose solution ........................................................................4.0mL

Thymine solution .......................................................................2.0mL

D-Alanine solution......................................................................2.0mL

Streptomycin sulfate solution ....................................................1.6mL

pH 7.2 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

Glucose ....................................................................................... 50.0g

Preparation of Glucose Solution: Add glucose to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster

ilize.

Thymine Solution:

Composition per 10.0mL:

Thymine ........................................................................................ 0.1g

Preparation of Thymine Solution: Add thymine to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

D-Alanine Solution:

Composition per 10.0mL:

D-Alanine....................................................................................... 0.1g

Preparation of D-Alanine Solution: Add D-alanine to distilled/de

ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize.

Streptomycin Sulfate Solution:

Composition per 10.0mL

Streptomycin sulfate ..................................................................... 2.5g

Preparation of Streptomycin Sulfate Solution: Add streptomy

cin sulfate to distilled/deionized water and bring volume to 10.0mL.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add agar, tryptose, NaCl, and beef extract

to distilled/deionized water and bring volume to 400.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add 4.0mL of the sterile glucose solution, 2.0mL of the sterile

thymine solution, 2.0mL of the sterile alanine solution, and 1.6mL of the

sterile streptomycin sulfate solution. Mix thoroughly. Aseptically distrib

ute into sterile tubes or flasks.

Use: For the cultivation of Bacillus subtilis.

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TBAB 298 Medium (Tryptose 298 Blood Agar Base Medium)